Video Cont.

 

Assembly of minicolony development video from still images:

1) Cells are inoculated in microtiter wells as described in basic protocol.

2) 14 hrs after inoculation, a Nikon TEM inverted microscope was focused on a  single minicolony with a 20X objective.  It is much easier to focus reliably on a small cluster of cells in this system than on a single cell.
 
3) Images are captured using a standard digital camera attached to the microscope and also connected to a computer.  We use a Canon Powershot G5 digital camera controlled by GBTimelapse software (Granite Bay Software) to capture images every 10 min for 5-10 days. Video was created from this series of images in Adobe® Premiere Pro CS4 and After Effects CS4.  After the video was assembled, variation in luminescence between frames was reduced using VirtualDub (1.3).

 

Citation